KMID : 0880220140520100819
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Journal of Microbiology 2014 Volume.52 No. 10 p.819 ~ p.824
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Citrimicrobium luteum gen. nov., sp. nov., aerobic anoxygenic phototrophic bacterium isolated from the gut of a sea cucumber Stichopus japonicus
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Jung Hong-Joo
Cha In-Tae Yim Kyung-June Song Hye-Seon Cho Ki-Chul Kim Dae-kyung Lee Hae-Won Lee Jae-Kook Seo Myung-Ji Roh Seong-Woon Lee Sung-Jae
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Abstract
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A Gram-stain negative, yellow-pigmented, motile, pleomorphic bacterium, designated strain CBA4602T, was isolated from the gut of the sea cucumber Stichopus japonicus, which was collected from Jeju Island in the Republic of Korea. In a phylogenetic analysis based on the 16S rRNA gene, strain CBA4602T belonged to the order Sphingomonadales in the class Alphaproteobacteria. The 16S rRNA gene sequence similarity between strain CBA4602T and ¡®Citromicrobium bathyomarinum¡¯ JF-1, the most closely related strain having nonvalidly published name, was 98.4%, followed by 95.2?96.7% identities with sequence of the other closest strains in the genus Erythrobacter. Strain CBA4602T had bacteriochlorophyll a and carotenoids. Strain CBA4602T grew in 0?10% (w/v) NaCl, at 10?42¡ÆC and pH 6.0?8.0, with optimal growth in 1?2% NaCl, at 30?37¡ÆC and pH 7.0. Strain CBA4602T was positive for catalase and oxidase activities and was able to hydrolyse gelatine and Tween 20 and 40, but not starch, Tween 80 or l-tyrosine. The G+C content of genomic DNA from strain CBA4602T was 68.0 mol% and Q-10 was the major detected isoprenoid quinone. The polar lipids were three unidentified phospholipids, three unidentified glycolipids, and two unidentified lipids. The dominant fatty acids were anteiso-C15:0, C16:0, anteiso-C17:0 and C18:0. As considering the current taxonomic status of the genus ¡®Citromicrobium¡¯ and polyphasic taxonomic analyses, strain CBA4602T represents a novel genus and species. The name Citrimicrobium luteum is proposed for the type strain CBA4602T (=KACC 17668T =JCM 19530T).
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KEYWORD
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Citrimicrobium luteum, sea cucumber, Stichopus japonicus, gut bacterium, polyphasic taxonomy
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